Research and Funding

Aged Rodent Colonies Handbook

Barrier Environmental Information

  Harlan:
Hybrid & BN rats
Charles River:
Mice, CR colony
Taconic Farms:
F344, Mutant Mice
Temperature 72° – 77° F 67°– 73° F 68°– 72° F
Humidity 40-55% 35 – 55% 30-70%
Water pH 5.8-6.0, 7-8 ppm chlorine pH 7.0-7.5, 4-6 ppm chlorine pH 6-8, 4-10 ppm chlorine
Light/Dark
Cycle
12 hr./12 hr. 12 hr./12 hr. 12 hr./12 hr.
Bedding Sterilized Aspen (BN, F344BN) (Harlan Product T.7083) or Tek-Fresh (mice, F344) (Harlan product T.7099) Wood shavings Sani-chip wood shavings
Feed Sterilized NIH31 or NIH31-Fortified (CR animals only) Sterilized NIH31 or NIH31-Fortified (CR animals only) Sterilized NIH31
Caging Aging – 3 rats or 5 mice per cage; CR – singly housed Aging – 5 mice per cage; CR – singly housed 3-5 rats per cage

NIA Diet Information (PDF, 15.75K)