Research and Funding

Aged Rodent Colonies Handbook

Barrier Environmental Information

  Charles River:
Hybrid & BN rats
Charles River:
Mice, CR colony
Temperature 72° – 77° F 67°– 73° F
Humidity 40-55% 35 – 55%
Water pH 5.8-6.0, 7-8 ppm chlorine pH 7.0-7.5, 4-6 ppm chlorine
Light/Dark
Cycle
12 hr./12 hr. 12 hr./12 hr.
Bedding Sterilized Aspen (BN, F344BN) (Harlan Product T.7083) or Tek-Fresh (mice, F344) (Harlan product T.7099) Wood shavings
Feed Sterilized NIH31 or NIH31-Fortified (CR animals only) Sterilized NIH31 or NIH31-Fortified (CR animals only)
Caging Aging – 2-3 rats per cage Aging – 5 mice per cage; CR – singly housed

NIA Diet Information (PDF, 16K)